Eukaryotic Pathogen CRISPR gRNA Design Tool

with (1) custom genome upload, (2) off-target analysis, (3) on-targets searching (for targeting gene families), (4) efficiency/activity prediction, (5) assisted oligo repair template design , (6) gRNA transcription problem identification, (7) flanking microhomology searching (for predicting deletions)

Please address any questions or suggestions (e.g. adding default genomes to our server) to Duo Peng at duopeng[at]
You can find available Eukaryotic pathogen genomes here