EuPaGDT

Eukaryotic Pathogen CRISPR guide RNA Design Tool

with (1) custom genome upload, (2) off-target analysis, (3) on-targets searching (for targeting gene families), (4) efficiency/activity prediction, (5) assisted oligo repair template design , (6) guide RNA transcription problem identification, (7) flanking microhomology searching (for predicting deletions)


batch mode available here

Gene tagging batch mode available here (?)

Our CRISPR/Cas9 gRNA design tool has a new look!!

Job Name:

RNA guided nuclease selection: (?)

choose one to automatically configure gRNA and PAM settings for each nuclease

SpCas9
SaCas9
custom (please manually enter PAM requirement)

Additional option settings: (click to expand a category)

guide RNA search parameters


guide RNA length:  

PAM motif(s):
(use comma to separate multiple PAM motifs
use IUPAC code for degenerate base)

on-target search parameters

(Default settings
recommended for most users)


homology arm length:
identity:    
coverage:   

off-target search parameters

(Default settings
recommended for most users)


seed sequence length (including PAM):

maximum number
of mismatches:

off-target PAM motif(s)(?):
(comma separated if more than one,
use IUPAC code for degenerate base)

HDR repair template parameters

(Default settings
recommended for most users)


homology arm length:(?)     

insertion:(?)    

Genome (expand a category and choose one):

genome naming paradigm:
specie strain database-version

Sequence:

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